b-Amyloid 1-40

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 and 43aa peptides (beta amyloid 1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synucleins is identical with non- Ab component (NACP) of AD. The 134aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140aa SYN-1 and 149aa SYN-2) and a small form (SYN-3, 42aa) are produced by alternative splicing. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:50000 \nELISA: 1:10,000-1:100,000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2275-74L

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Borchelt, D.R., et al., (1997) Neuron 19:939-945. 2. Savage, M.J., et al., (1998) J. Neurosci. 18:1743-1752. 3. Vassar, R., et al., (1999) Science 286:735-741. 4. Ulery, P.G., et al., (2000) J. Biol. Chem. 275:7410-7415.

Additional Info

Recognizes human b-amyloid. Demonstrates a high degree of homology with human and murine b-amyloid (1-40) and b-amyloid (1-42). Weak crossreactivity is observed with b-amyloid (1-28) and b-amyloid (12-28). No crossreaction is observed with CGRP. Species crossreactivity: mouse.