b-Amyloid 1-42

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 aa peptides (beta amyloid1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140 aa a-synucleins is identical with non-Ab component (NACP) of AD. The 134 aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1;1000-1:5000\nELISA: 1:10,000-1:100,000. Coat with 50-100ng of A2275-74P Control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2275-75T

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Neve, R., et al. (1992) PNAS 89: 3448. 2. Goldgaber, D. et al. (1987) Science 235: 877. 3. Yankner, B., et al. (1989) Science 245: 417. 4. Golde, T. et al. (1992) Science 255: 728. 5. Wang, R., et al. (1991) J. Biol. Chem. 266: 16960. 6. Shigematuse, K., et al. (1992) J. Neurosci. 31: 443. 7. Kang, J. et al. (1987) Nature 325: 733. 8. Tanzi, R., et al. (1987) Science 235: 880. 9. Weidmann, A., et al. (1989) Cell 57: 115.

Additional Info

Recognizes human b-amyloid 1-42. Minimal crossreactivity with b-1-40. Species sequence homology: mouse b-1-42.