This antibody may be used for detection of beta-galactosidase expressed by the E.coli lacZ gene encoded in many cloned gene sequences, and serves as an indicator for fusion proteins encoded by an inserted DNA sequence.
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 2.5-5g/ml
Immunoprecipitation: 2ug/mg protein lysate
Optimal dilutions to be determined by the researcher.
Positive Control:
Purified recombinant b-galactosidase protein or transfected cell lines expressing b-galactosidase-tagged proteins
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.