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b-Galactosidase (Beta-galactosidase, Beta-gal) (Biotin)

Cat no: G1041-02K


Supplier: United States Biological
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Biotinylated b-galactosidase is prepared from an enzyme of exceptionally high specific activity isolated from a special strain of E. coli. Unlike mammalian b-galactosidase, the activity of this E. coli enzyme is optimal at an alkaline pH, enabling it to be used free of interference from endogenous enzyme activity. For many applications, Avidin D followed by biotinylated b-galactosidase affords a highly sensitive system which can be used in a variety of enzyme immunoassays, including screening for monoclonal antibodies. One unit is 1 micromole o-nitrophenyl-b-D-galacto-pyranoside hydrolyzed per min. at 37 degrees C, pH 7.3. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Activity: 746 units/mg protein Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G1041-02K
Applications: ELISA
Conjugates: Biotin
Size: 100U
Form: Supplied as a powder in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide. Reconstitute with 1ml dH2O. Labeled with Biotin.
Alternative names: EC=3.2.1.23, b-gal

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