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b-Galactosidase (E. coli) (FITC)

b-Galactosidase (E. coli) (FITC)

Cat no: G1041-05R

Supplier: United States Biological
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Beta-galactosidases are widespread in microorganisms, animals and plants. That from the Escherichia coli strain K12 has been particularly studied at Anfinsen's laboratory in connection with genetic experiments on gene regulation of protein synthesis. Beta-galactosidase is tetrameric, being composed of four identical subunits of 135kD, each with an active site which may be independently active (Melcher and Messe 1973). The enzyme is readily fragmented into small peptides (Marinkovic et al. 1975). The amino acid analysis indicates approximately 1170 residues per subunit (Fowler and Zabin 1970). See also Langley et al. (1975) and Naider et al. (1972). Kaneshiro et al. (1975) report on an active dimer. Applications: Suitable for use in Immunofluorescence, FLISA and Western Blot. Other applications not tested. Recommended Dilution: FLISA: >1:20,000 Immunofluorescence (IC): 1:500-1:2500 Western Blot: 1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Catalogue number: G1041-05R
Hosts: Rabbit
Applications: ELISA, Immunofluorescence, Western Blot
Size: 1mg
Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with Fluorescein Isothiocynate (FITC).
P type: Pab
Isotype: IgG
Purity: Purified
Alternative names: EC=3.2.1.23, b-gal
References: 1.Peterson KR et al. Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 39:1-9 (2004). The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970).
Additional info: Recognizes beta-galactosidase

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