b-Glucosidase, Almond

Catalyzes the hydrolysis of glucosides. Emulsin originally was the term applied to the extract of sweet almonds. It remains as the historical prototype of b-glucosidase. Many other sources of the enzyme have been described.\n\nEmulsin also shows significant D-galactosidase activity and there is some question as to whether or not it is one enzyme with two activities, because non-parallel response to inhibitors points to two separate enzymes. D-mannosidase has also been reported in almond emulsin.\n\nReaction Equation: \nb-D-Glucosidase + H2 = Alcohol + D-Glucose \n\nContaminants: \na-Amylse (same/less than) 0.01% \n\nActiivity:\nAs reported \n\nSpecific Activity: (same/more than) 30u/mg protein \n\nApplications:\nSuitable for use as calibrator or control serum. Other applications have not been tested.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3059-01

Size

10KU

Form

Supplied as lyophilized powder.

Purity

Purified

References

1. Anderson, F., Cunningham, W., and Manners, D.: Studies on Carbohydrate-Metabolizing Enzymes. 10. Barley[[beta]]-Glucosidases, Biochem. J., 90, 30 (1964). 2. Brady, R., O'Brien, J., Bradley, R., and Gal, A.: Sphingolipid Hydrolases in Brain Tissue of Patients with Generalized Gangliosidosis, Biochim. Biophys. Acta, 210, 193 (1970). 3. Chakrabarti, A., and Storey, K.: Enhanced Glucose Production from Cellulose Using Coimmobilized Cellulase and [[beta]]-Glucosidase, Appl. Biochem. Biotech., 22, 263 (1989). 4. Dale, M., Ensley, H., Kern, K., Sastry, K., and Byers, L.: Reversible Inhibitors of [[beta]]-Glucosidase,Biochem., 24, 3530 (1985). 5. Dale, M., Kopfler, W., Chait, I., and Byers, L.: [[beta]]-Glucosidase: Substrate, Solvent, and ViscosityVariation as Probes of the Rate-Limiting Steps, Biochem., 25, 2522 (1986). 6. Eberhart, B., and Beck, R.: Localization of the [[beta]]-Glucosidases in Neurospora crassa, J. Bacteriol.,101, 408 (1970). 7. Fleming, L., and Duerksen, J.: Purification and Characterization of Yeast [[beta]]-Glucosidases, J. Bacteriol., 93, 135 (1967). 8. Gatt, S., and Rapport, M.: Isolation of [[beta]]-Galactosidase and [[beta]]-Glucosidase from Brain, Biochim Biophys. Acta, 113, 567 (1966). 9. Guilbault, G., and Kramer, D.: An Electrochemical Method for the Determination of Glucosidase and Mercuric Ion, Anal. Biochem., 18, 313 (1967). 10. Guilbault, G., Sadar, M., and Peres, K.: Fluorometric Determination of Carbohydrates, Anal. Biochem., 31, 91 (1969). 11. Han, Y., and Srinivasan, V.: Purification and Characterization of [[beta]]-Glucosidase of Alcaligenes faecalis, J. Bacteriol., 100, 1355 (1969). 12. Helferich, B., and Kleinschmidt, T.: Hoppe-Seyler's Z. Physiol. Chem., 340, 31 (1965). 13. Hildebrand, D., and Schroth, M.: [[beta]]-Glucosidase Activity in Phytopathogenic Bacteria, Appl. Microbiol., 12, 487 (1964). 14. Hultberg, B., and Ockerman, P.: [[beta]]-Glucosidase Activities in Human Tissues. Findings in Gaucher's Disease, Clin. Chem. Acta, 28, 169 (1970). 15. Hyun, J., Misra, R., Greenblatt, D., and Radin, N.: Synthetic Inhibitors of Glucocerebroside [[beta]]-Glucosidase, Arch. Biochem. Biophys., 169, 364 (1974). 16. Kanfer, J., Raghaven, S., and Mumford, R.: Hydrolytic and Transglucosylation Activities of a Purified Calf Spleen [[beta]]-Glucosidase, Biochim. Biophys. Acta, 391, 129 (1975). 17. Kanfer, J., Raghaven, S., Mumford, R., Labow, R., Williamson, D., and Lyne, D.: Deficiency of Steroid [[beta]]-Glucosidase in Gaucher Disease, Biochem. Biophys. Res. Comm., 67, 683 (1975). 18. Kaplan, J., and Tacreiter, W.: The [[beta]]-Glucosidase of the Yeast Cell Surface, J. Gen. Physiol., 50, 9 (1966). 19. Kelemen, M., and Whelan, W.: Inhibition of Glucosidases and Galactosidases by Polyols, Arch. Biochem. Biophys., 1217, 423 (1966). 20. Kraml, J., Kolinsk

Alternative Names

b-D-Glucosidase, Almond, b-D-Glucoside glycohydrolase (EC 3.2.1.21)