B7H4 (B7-H4, B7S1, B7x, BC032925, Immune Costimulatory Protein B7H4, MGC41287, PRO1291, T Cell Costimulatory Molecule B7x, V Set Domain-containing T Cell Activation Inhibitor 1, VCTN1)

B7H4, also known as B7x, is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7H4 deficient mice are only minimally affected; suggesting B7H4 is important in the fine tuning of the T-cell mediated immune response. B7H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC).\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nFlow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul.\nImmunohistochemistry (Paraffin): 1:50. Does not require digestion pretreatment of paraffin sections. Does not require heat-treatment prior to staining paraffin sections.\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nImmunohistochemistry Positive Control: \nTonsil, breast carcinoma\n\nRecommended Negative Control:\nI1904-78R: IgG1 Murine Negative Control\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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