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BACE1, Ectodomain (Beta-site APP Cleaving Enzyme)

BACE1, Ectodomain (Beta-site APP Cleaving Enzyme)

Cat no: B0002-91L

Supplier: United States Biological
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Cleavage of amyloid precursor protein (APP) into 40 or 42 amino acid amyloid beta (ab) peptides is a result of the action of two proteases beta- and gamma-secretase. The beta-site APP cleaving enzyme, or BACE1, is a transmembrane aspartic protease that is localized in the golgi and endosomes. Low levels of BACE1 mRNA are found in most adult tissues with a higher level of expression in pancreas. Applications: Suitable for use in ELISA, Immunoprecipitation and Neutralization. Other applications not tested. Recommended Dilutions: ELISA: Use in a Direct ELISA. Immunoprecipitation: 25ug/ml to immunoprecipitate BACE-1 from conditioned media of cell culture spike with recombinant human BACE1 or recombinant mouse BACE1. Western Blot after Immunoprecipitation. Neutralization: The Neutralization Dose (ND50) is typically 10ug/ml. It is measured by the ability of the antibody to neutralize recombinant human BACE1 or recombinant mouse BACE1 cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10uM). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B0002-91L
Reactivities: Human, Mouse
Hosts: Mouse
Applications: ELISA, Immunoprecipitation
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
Alternative names: ASP2, BACE, EC=3.4.23.46, FLJ90568, HSPC104, KIAA1149, Memapsin-2
Additional info: Recognizes human BACE1 ectodomain. Species Crossreactvity: mouse. Does not react with recombinant human or mouse BACE2, recombinant human ADAM8, ADAM9, ADAM10, ADAM15 or TACE.

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