Baculovirus cassette vector pAc-l-Fc for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fab antibody gene fragments.
Vector Size:
6701bp
Resistance:
Vector contains an ampicillin resistance marker gene
Applications:
Suitable for use in cloning of heavy and light chain variable domain genes of scFv or Fab antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries.
Cloning sites:
XhoI and SpeI for heavy chain Fab fd genes SacI and EcoRV for light chain Fab(l) genes. Expression of immunoglobulin heavy and light chains in insect cells and secretion of assembled IgG(l) antibodies into the supernatant.
Storage and Stability:
For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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