Bad, phosphorylated (Ser136) (Bcl2 Antagonist of Cell Death)

Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-xL. Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK and mitochondria-anchored PKA. Phosphorylation of Ser155 by PKA plays a critical role in blocking the dimerization of Bad to Bcl-xL because of its position in the BH3 domain.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500. Diluted antibody in TBS, 5% BSA, 0.1% Tween-20. Incubate at 4 degrees C overnight with gentle shaking.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0003-58

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Yang, E., et al. (1995) Cell 80: 285-291. 2. Zha, J., et al. (1996) Cell 87: 619-628. 3. Datta, S.R., et al. (1997) Cell 91: 231-241. 4. Mizushima, S. and Nagata, S. (1990) Nucleic Acids Res. 18: 5322. 5. Peso, L., et al. (1997) Science 278: 687-689. 6. Bonni, A., et al. (1999) Science 286: 1358-1362. 7. Tan, Y., et al. (1999) J. Biol. Chem. 274: 34,859-34,867. 8. Harada, H., et al. (1999) Mol. Cell 3: 413-422. 9. Tan, Y., et al. (2000) J. Biol. Chem. 275: 25,865-25,869. 10. Lizcano, J. (2000) Biochem. J. 349: 547-557. 11. Datta, S., et al. (2000) Mol. Cell 6: 41-51. 12. Jamieson, C.A. and Yamamoto, K.R. (2000) Proc. Natl. Acad. Sci. USA 97: 7319-7324. 13. Laine, J., et al. (2000) Mol. Cell 6: 395-407. 14. Tan, Y., et al. (1999) J. Biol. Chem. 274: 34,859-34,867.

Additional Info

Detects transfected levels of Bad only when phosphorylated at Ser136. Does not detect non-phosphorylated Bad or Bad phosphorylated at other sites. Does not detect related family members. Species crossreactivity: mouse. Expected to crossreact with human and rat based on 100% sequence homology.