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BAFF Receptor (BAFFR, BAFF-R, B-cell-activating Factor Receptor, BLyS Receptor 3, BR3, BROMIX, CD268, CVID4, MGC138235, Prolixin, Tumor Necrosis Factor Receptor Superfamily Member 13C, TNFRSF13C) (Biotin)

BAFF Receptor (BAFFR, BAFF-R, B-cell-activating Factor Receptor, BLyS Receptor 3, BR3, BROMIX, CD268, CVID4, MGC138235, Prolixin, Tumor Necrosis Factor Receptor Superfamily Member 13C, TNFRSF13C) (Biotin)

Cat no: B0003-68Y2

Supplier: United States Biological
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Human CD268 (BAFFR, BAFF receptor) is a type I TNF superfamily receptor member #13c. It is highly specific for CD257 (BAFF), which itself does bind to other receptors TACI, and BCMA. CD268 expressed on B cells and its ligation by CD257 (BAFF) regulates maturation (1). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow CytometryProtocol: 1. Wash 5x10e5 cultured human Raji cells per tube and incubated 45 minutes on ice with 80ul of B0003-68Y2 at 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 3. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml B0003-68Y. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B0003-68Y2
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Conjugates: Biotin
Size: 100ug
Form: Supplied as liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5% gentamicin sulfate. Labeled with Biotin.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1) Thompson, J S, C Ambrose, et al. (2001) Science 293(5537): 2108-2111.
Additional info: Recognizes human CD268. Binds to recombinant CD268 (but not CD269) in ELISA and native CD268 expressed on Raji cell surface.

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