5'-G^G A T C C-3'
3'-C C T A G^G-5'
Source: Bacillus amyloliquefaciens H
Supplied with:
B0070A: Reaction Buffer (10X) for BamHI+, 10X
Supplied as a liquid in10mM Tris-HCl, pH 8.0, 5mM MgCl2, 100mM KCl, 0.02% Triton X-100, 0.1mg/ml BSA. Supplied with 2x1ml of Reaction Buffer (10X).
Concentration: 10u/ul
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-Bsp120I fragments in 1 hour at 37 degrees C in 50ul of assay buffer.
Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
Storage Buffer: 10mM Tris-HCl, pH 7.4 at 25C, 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Incubation Temp: 37 degrees C
Methylation Effects:
BamHI does not cut GGATm4CC, GGATm5CC, GGAThm5Chm5C, GGAhm5UCC, but cuts GGATCm5C, GGm6ATCC, GGm6ATCm5C and GGATCm4C.
Number of Recognition Sites in DNA Molecules:
Lambda: 5
PhiX174: 1
M13mp18/19: 1
pBR322: 1
pUC18/19: 1
pUC57: 1
pTZ19R/U: 1
Stability during Prolonged Incubation: A minimum of 0.5 units of enzyme are required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degrees C.
Thermal Inactivation: Only small amounts of enzyme (up to 10 units) can be inactivated at 80 degrees C for 20min.
Compatible Ends: BclI, BglII, Bsp143I, MboI, PsuI
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