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Basal Bodies of Cillia

Basal Bodies of Cillia

Cat no: B0099-85

Supplier: United States Biological
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Flagella and cillia both project from the cell surface and beat in distinctive patterns. Flagella are whiplike tails that propel many free-living cells, such as sperm, through fluid environments. Cilia are shorter and usually more profuse than flagella. In both flagella and cilia, nine pairs of microtubules ring two central microtubules. This arrangement is called a "9 + 2 array." A system of spokes and links holds the arrangement together. Microtubules of a flagellum or cilium arise from centrioles, which remain at the base of the completed structure as a basal body. These are sites of dense material that generate large numbers of microtubules. Applications: Suitable for use in Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B0099-85
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2.
P type: Mab
Isotype: IgG1
Purity: Mouse ascites
References: Comer MT et al. Application of a marker of ciliated epithelial cells to gynaecological pathology. J Clin Pathol 52:355-7 (1999). <PUBMED:10560355> Comer MT et al. Expression of an antigen associated with basal bodies of human ciliated epithelial cells. Histochem J 31:39-43 (1999). <PUBMED:10405821> Comer MT et al. Induction of a differentiated ciliated cell phenotype in primary cultures of Fallopian tube epithelium. Hum Reprod 13:3114-20 (1998). <PUBMED:9853867>
Additional info: This antibody recognizes the basal bodies of cillia in all cilliated cells. Species Crossreactivity: Crossreacts with Human.

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