Basal Bodies of Cillia

Flagella and cillia both project from the cell surface and beat in distinctive patterns. Flagella are whiplike tails that propel many free-living cells, such as sperm, through fluid environments. Cilia are shorter and usually more profuse than flagella. In both flagella and cilia, nine pairs of microtubules ring two central microtubules. This arrangement is called a "9 + 2 array." A system of spokes and links holds the arrangement together. Microtubules of a flagellum or cilium arise from centrioles, which remain at the base of the completed structure as a basal body. These are sites of dense material that generate large numbers of microtubules.\n\nApplications: \nSuitable for use in Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0099-85

Size

100ug

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Mouse ascites

Isotype

IgG1

References

Comer MT et al. Application of a marker of ciliated epithelial cells to gynaecological pathology. J Clin Pathol 52:355-7 (1999). <PUBMED:10560355>\nComer MT et al. Expression of an antigen associated with basal bodies of human ciliated epithelial cells. Histochem J 31:39-43 (1999). <PUBMED:10405821>\nComer MT et al. Induction of a differentiated ciliated cell phenotype in primary cultures of Fallopian tube epithelium. Hum Reprod 13:3114-20 (1998). <PUBMED:9853867>

Additional Info

This antibody recognizes the basal bodies of cillia in all cilliated cells. Species Crossreactivity: Crossreacts with Human.