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Bcl-2 (Apoptosis Regulator Bcl-2, BCL2)

Bcl-2 (Apoptosis Regulator Bcl-2, BCL2)

Cat no: B0807-02F2

Supplier: United States Biological
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Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects Bcl-2 in cells and tissues Immunohistochemistry (FFPE): 0.4-1ug/ml Immunohistochemistry (Frozen): 0.4-1ug/ml in formalin- acetone fixed tissues Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B0807-02F2
Reactivities: Human
Hosts: Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1ml sterile PBS.
P type: Mab
Isotype: IgG1
Purity: Purified by immunoaffinity chromatography.
References: 1. Ngan, B. et al., New Eng. J. Med. 318:1638-1644 (1988) 2. Hockenbery D., et al., Nature 348: 334-336 (1990).
Additional info: Recognizes human Bcl-2.

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