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Bcl-2 (B Cell Leukemia 2)

Cat no: B0807-06C


Supplier: United States Biological
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Apoptosis regulator Bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. At least 2 isoforms (alpha and beta) are produced by alternate splicing with different C-terminal ends. Applications: Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (formalin, paraffin, and frozen): 0.4-1.0ug/ml Antigen retrieval may improve the staining. Optimal dilution determined by the researcher. Positive control: Follicular and diffuse large B cell lymphoma tumors Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B0807-06C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
P type: Mab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes human Bcl-2.

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