Apoptosis, the programmed cell death, is the process that eliminates damaged cells without evoking inflammation. Bcl-2 and related cytoplasmic proteins are key regulators of apoptosis. At least 15 Bcl-2 family members have been identified in mammalian cells and several others in viruses. All members possess at least one of four conserved motifs known as Bcl-2 homology domains. Most pro-survival members, which can inhibit apoptosis in face of a wide variety of cytotoxic insults, contain at least HB1 and BH2. The two pro-apoptosis subfamilies differ markedly in their relatedness to Bcl-2. Bax, Bak and Bok (also call Mtd), which contain BH1, BH2, and BH3, resemble Bcl-2 fairly closely. In contrast, the seven other known mammalian "killers" possess only the central short BH3 domain; they are otherwise unrelated to any known protein, and only Bik and Blk are similar to each other. These "BH3 domain" proteins may well represent the physiological antagonists of the survival proteins.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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