Defensin beta 1 (BD1) is a secreted cationic antimicrobial peptide and member of the beta-defensin family, expressed at epithelial surfaces, which plays an important role in innate immunity. Studies have shown that the activity of BD-1 is compromised in patients with Cystic Fibrosis due to salt-dependent inactivation.
Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 0.25-1ug/ml
Western Blot: 0.1 -0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.