Defensin beta 3 (BD-3), a cationic non-haemolytic antimicrobial peptide and member of the beta-defensin family, highly expressed by skin and tonsils, which plays an important role in the innate immune response to potentially pathogenic microbes, including multiresistant S. aureus and vancomycin-resistant E. faecium.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5- 2ug/ml
Western Blot: 0.1- 0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.