Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36aa), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. beta-Defensins are 3-5kD peptides ranging in size from 33-47aa. Recombinant Human BD-3 is a 5.1kD protein containing 45aa.
Source:
Recombinant protein corresponding to human BD3, expressed in E. coli.
Biological Activity:
Exhibits antimicrobial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E.coli.
Endotoxin Level:
<0.1ng/ug (1EU/ug)
AA Sequence:
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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