BD4 (beta Defensin-4)

Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.\n\nThe genes encoding human a and b -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a-and b-based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a -defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b -defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.\n\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nB0903-20 BD4, Mouse Control Peptide (beta Defensin-4) \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0903-25

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

(1) Jia HP et al (1999) gene accession # AF155882; Harder et al (1997) Nature 387, 861; Ganz T (1999) Science 286, 420; Yang D et al (1999) Science 286, 525.

Additional Info

Recognizes mouse defensin-4. No significant homology exists with human beta-defensins-4 or other defensins.