BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247aa polypeptide precursor, which also contains a signal sequence of 18aa residue and a propeptide of 110aa. Recombinant human BDNF is a 27kD homodimer of two 119aa subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical. Manufactured using all non-animal reagents.
Source:
Recombinant protein corresponding to human BDNF, expressed in E.coli.
Biological Activity:
Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1ug/ml.
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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