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Beclin-1 (Coiled-coil Myosin-like Bcl-2-Interacting Protein, ATG6, BECN1, GT197, VPS30)

Beclin-1 (Coiled-coil Myosin-like Bcl-2-Interacting Protein, ATG6, BECN1, GT197, VPS30)

Cat no: B0981-23G

Supplier: United States Biological
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The Beclin-1 (BECN1) gene encodes a 60kD coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity and tumorigenesis. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:50 Immunocytochemistry: 1: 50 Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Positive Control: Antigen standard for BECN1 (NM_003766) (transient overexpression lysate) Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B0981-23G
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.2, 0.15M sodium chloride, 0.05% BSA, 40% glycerol, 0.01% sodium azide.
P type: Mab
Isotype: IgG
Purity: Rabbit ascites
Alternative names: anti-ATG 6 antibody, anti-ATG6 antibody, anti-BECN 1 antibody, anti-BECN1 antibody, anti-Beclin1 antibody, anti-Coiled coil myosin like BCL2 interacting protein antibody, anti-GT197 antibody, anti-Protein GT197 antibody, anti-VPS 30 antibody, anti-VPS30 antibody
References: 1. Aita VM, et al. Genomics 59(1):59-65, 1999 2. Liang XH, et al. Nature 402(6762):672-6, 1999 3. Liang XH, et al. Cancer Res 61(8):3443-9, 2001
Additional info: Recognizes human Beclin-1 (Coiled-coil Myosin-like Bcl-2-Interacting Protein, ATG6, BECN1, GT197, VPS30). Species Crossreactivity: Does not react in Mouse or Rat.

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