Beclin-1, NT (Coiled-coil Myosin-like Bcl-2-Interacting Protein)

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be \ndefective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2- interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can \ninhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and \nmammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6)\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: .5-1ug

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SPECIFICATIONS

Catalog Number

B0981-25

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.\n

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n

Isotype

IgG

References

1. Gozuacik D and Kimchi A. Oncogene. 2004; 23:2891- 906. 2. Kisen GO, et al Carcinogenesis 1993; 14:2501- 5. 3. Liang XH, Ket al J. Virol. 1998; 72:8586-96. 4. Kametaka S, et al J. Biol. Chem. 1998; 273:22284-91. 5. Liang XH,et al Nature 1999; 402:672-6. 6. Pattingre S, et al Cell 2005; 122:927-39. (WD1005)

Additional Info

Recognizes human Beclin-1. Species Crossreactivity: mouse and rat.