The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta-lactams are structurally related through the presence of a core beta-lactam ring. Bacterial resistance to beta-lactams continues to increase, primarily due to the production of beta-lactamases. Beta-lactamases catalyze the hydrolysis of the beta-lactam bond which destroys antibacterial activity. Bacteria that produce TEM-or SHV-type beta-lactamases have point mutations in structural genes that have extended the substrate speci?city of these beta-lactamases. As a result, many of the beta-lactamase-producing Gram-negative pathogens have become multi-drug resistant.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 10-20ug/ml
Western Blot: 10ug/ml detects a band at ~31.5kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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