5'-A^G A T C T-3'
3'-T C T A G^A-5'
Enzyme Properties:
Methylation Effects:
Dam: completely overlaps-no effect.
Dcm/CpG/EcoKl: never overlaps-no effect.
EcoBl: may overlap-cleavage impaired.
Stability during Prolonged Incubation:
A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degrees C.
Thermal Inactivation:
Enzyme is not inactivated by incubation at 80 degrees C for 20 minutes.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends:
BamHI, BclI, Bsp143I, MboI, PsuI
Number of Recognition Sites in DNA:
Lambda: 6
PhiX174: 0
M13mp18/19: 1
pBR322: 0
pUC18/19: 0
pUC57: 0
pTZ19R/U: 0
Quick Search
