BHMT (Betaine-homocysteine methyltransferase, BHMT)

Cat no: B1082-13

Supplier: United States Biological

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e encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:16,000 Western Blot: 0.03-0.1ug/ml ~45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Immunohistochemistry: 2-4ug/ml In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Other dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See B1082-13-P. Antibody

Catalogue number:	B1082-13
Reactivities:	Human, Mouse, Rat
Hosts:	Goat
Applications:	ELISA, Immunohistochemistry, Western Blot
Size:	100ug
Form:	Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by peptide affinity chromatography.
Alternative names:	betaine-homocysteine methyltransferase; BHMT
References:	1. Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice., Collinsova M, Strakova J, Jiracek J, Garrow TA., J Nutr. 2006 Jun;136(6):1493-7.
Additional info:	Recognizes human BHMT. Species Crossreactivity: mouse, rat. Species sequence homology: canine

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BHMT (Betaine-homocysteine methyltransferase, BHMT)

Cat no: B1082-13

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