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BID (BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist)

BID (BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist)

Cat no: B1095-03F

Supplier: United States Biological
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Alternate Name: BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist Application(s): Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 26+24kD bands observed in Human fibroblast A431 lysates (calculated MW of 26.8kD according to NP_932070.1 and 22.0kD according to NP_001187.1). Recommended concentration: 0.1-0.3ug/ml. 0.1ug/ml staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See B1095-03F-P. Antibody
Catalogue number: B1095-03F
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: Lund T, Stokke T, Olsen OE, Fodstad O. , Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein Bim(EL) and induces apoptosis., Br J Cancer. 2005 Apr 12; [Epub ahead of print]
Additional info: This antibody is expected to recognize all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1)

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