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BID, Full Length (BH3 Interacting Domain Death Agonist)

BID, Full Length (BH3 Interacting Domain Death Agonist)

Cat no: B1096-02

Supplier: United States Biological
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The BH3 domain-only protein BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID and for the subsequent release of cytochrome c during apoptosis is unclear. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B1096-02
Reactivities: Mouse
Hosts: Rabbit
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A and immunoaffinity chromatography.
References: 1. Wang, K. et al. (1996) Genes Dev. 10, 2859
Additional info: Detects endogenous levels of total full length mouse BID. Species crossreactivity: Mouse specific.

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