Differentiation of Group D from non-Group D Streptococci.
Description:
Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Gram positive bacteria other than enterococci are inhibited by the bile salts.
Storage:
Store sealed bottle containing dehydrated medium at 2 to 30 degrees C. Once opened and recapped, place container in low humidity environment at same storage temperature. Protect from moisture and light. Dehydrated medium should be discarded if not free flowing or if color has changed from original light beige color.
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