Custom Immunoassay Development Services:
United States Biological offers a comprehensive menu of Custom Immunoassay Development Services for research procedures. In particular, we have extensive experience in developing immunoassay kits for quantitative detection of enzymes, antibodies, antigens, receptors, inhibitors, peptides and small molecules from cell culture supernatant, tissue homogenate, cell lysate, serum, plasma, ascites, cerebrospinal fluid and urine. We can also build up kits that are intended for analysis of enzyme activity, protein-protein interaction, nucleic acid-protein interaction and inhibitor characterization.
We have established proprietary antibody screening and recombinant protein standards manufacturing methods that are tailored for immunoassay development. Our staff scientists follow rigorous guidelines for quality control with a focus on thorough optimization of every aspect of assay development, including antibody specificity, assay sensitivity, reproducibility (intra- and inter-assay), cross-reactivity, standard curve range, assay accuracy (linearity and recovery) and kit stability.
Our expertise allows us to fully accommodate your needs and ensure the reproducibility of the customized kits in your hands.
Immunoassay Formats:
1. Direct ELISA
2. Sandwich ELISA
3. Competitive ELISA
4. Immuno-capture enzymatic assay
5. Colloidal gold immuno-chromatography strip
Detection Methods:
1. Light absorbance
2. Fluorescence intensity or polarization
3. Fluorescence resonance energy transfer
4. Luminescence
Assay Milestones:
1. Feasibility assessment and assay design
2. Assay optimization
3. Assay Validation
4. Manufacturing
United States Biological is committed to quality and customer satisfaction. A dedicated project manager will be assigned to each project to interact with the customer to meet his specific requirements.
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