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Biotin anti-human TNF-alpha

Biotin anti-human TNF-alpha

Cat no: 502903


Supplier: BioLegend
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TNF-alpha is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-alpha. Monomeric human TNF-alpha is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Catalogue number: 502903
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence
Conjugates: Biotin
Size: 50 microg
Clone: MAb11
Gene: 7124
Dilutions: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate.For use as an ELISPOT detection antibody, a concentration range of 0.5-2.0 microg/ml is recommended. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: E. coli-expressed, recombinant human TNF-alpha
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
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