Biotin anti-mouse/rat TNF-alpha

Cat no: 516001

Supplier: BioLegend

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TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric mouse TNF-alpha is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The Poly5160 antibody reacts with mouse and rat tumor necrosis factor-alpha (TNF-alpha).

Catalogue number:	516001
Reactivities:	Mouse, Rat
Hosts:	Goat
Applications:	ELISA
Conjugates:	Biotin
Size:	4 plates
Clone:	Poly5160
Gene:	21926
Dilutions:	Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection, the suggested use of this reagent is 1:1000 dilution. To obtain a linear standard curve, serial dilutions of mouse TNF-alpha recombinant protein ranging from 4 to 500 pg/ml are recommended for each ELISA plate or serial dilutions of rat TNF-alpha recombinant protein ranging from 8 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	The antibody solution should be stored undiluted at 4C. Do not freeze.
Antigen:	Recombinant mouse TNF-alpha
Isotype:	Goat IgG
Clonality:	Polyclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C

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Biotin anti-mouse/rat TNF-alpha

Cat no: 516001

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