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Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)

Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)

Cat no: B1750-06A

Supplier: United States Biological
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This antibody has a very high affinity for biotin and is excellent for use in various amplification techniques. It recognizes both the free and protein- conjugated (either soluble or cell bound) form of biotin. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 2.5-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B1750-06A
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG1,k
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes biotin in all species.

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