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Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)

Cat no: B1750-38


Supplier: United States Biological
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Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:20,000 Optimal dilutions to be determined by the researcher. Hybridoma: 3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Culture Medium: RPMI 1640 with 10% fetal calf serum Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B1750-38
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein A/G affinity chromatography from culture supernatant.
References: 1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
Additional info: Recognizes and reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blot, after SDS-PAGE, reacts with biotinylated proteins in both reduced and non-reduced forms.

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