Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:20,000
Optimal dilutions to be determined by the researcher.
Hybridoma:
3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Culture Medium:
RPMI 1640 with 10% fetal calf serum
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.