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Products Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)
| Catalogue number: | B1750-38 |
| Hosts: | Mouse |
| Applications: | ELISA, Western Blot |
| Size: | 100ul |
| Form: | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%. |
| P type: | Mab |
| Isotype: | IgG2a,k |
| Purity: | Purified by Protein A/G affinity chromatography from culture supernatant. |
| References: | 1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York. |
| Additional info: | Recognizes and reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blot, after SDS-PAGE, reacts with biotinylated proteins in both reduced and non-reduced forms. |
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