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Biotin (FITC)

Biotin (FITC)

Cat no: B1750-01M

Supplier: United States Biological
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Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher. Absorption Wavelength: 494nm Emission Wavelength: 518nm Fluorochrome/Protein Ratio: 8.1 moles FITC per mole of Goat IgG Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: B1750-01M
Hosts: Goat
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Size: 1mg
Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA (IgG and protease free), 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes free biotin, coupled with KLH, BSA or IgG/Y. No reactivity to KLH or BSA.

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