HIAP belongs to a family of apoptosis inhibitor proteins that have a baculovirus IAP repeat (BIR). These BIR's interact with TRAF1 and TRAF2 that forms a heteromeric complex. This complex is what is thought to inhibit apoptosis. HIAP is located in the cytoplasm.
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500
Immunohistochemistry (paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Positive Control:
Daudi cell lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.