logo
logo
BL21 (DE3) Competent Cells
Bioline USA

Supplier:

Bioline USA

Cat no: BIO-85032

BL21 (DE3) Competent Cells

The BL21(DE3) Competent Cells are designed for high-level protein expression and easy induction using T7 promoter constructs. These strains are lysogens of bacteriophage DE3, a lambda derivative containing the gene for T7 RNA polymerase under control of the lacUV5 promoter. Induction with IPTG allows production of T7 RNA Polymerase which then directs the expression of the target gene located downstream of the T7 promoter in the expression vector. Each BL21 (DE3) strain provides varying degrees of regulation and expression control. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21 (DE3) cells than in BL21 (DE3) pLysS or BL21 (DE3) pLysE. Efficiency: >1 x 10e7cfu/ug

Prices direct from Bioline USA

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

BIO-85032

Size

1ml (10 x 100 micro l)

P Type

Cloning

Read more on Supplier website

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

Genovis introduces GlycINATOR™• Works on most IgGs• Effective on high-mannose and bisected N-linked Fc-glycans • Rapid – 30-minute...

Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...