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BL21 (DE3) pLysS Competent Cells
Bioline USA

Supplier:

Bioline USA

Cat no: BIO-85033

BL21 (DE3) pLysS Competent Cells

This strain contains the T7 RNA polymerase gene but also carries the plasmid pLysS that constitutively expresses T7 lysozyme, a natural inhibitor of T7 RNA Polymerase. This strain is used to minimize basal level expression of potentially toxic gene products before induction with IPTG. When induced with IPTG, the T7 RNA polymerase is produced in excess, overcoming the inhibition of T7 RNA polymerase by T7 lysozyme. Genotype: F- ompT hsdSB(rB-mB-) gal dcm (DE3) pLysS (CamR)

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SPECIFICATIONS

Catalog Number

BIO-85033

Size

1ml (10 x 100 micro l)

P Type

Cloning

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