This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:
Immunofluorescence: 20ug/ml
Western Blot: 0.5-1ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.