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Blk, active, Recombinant, Human (B lymphoid kinase, B lymphoid tyrosine kinase)

Blk, active, Recombinant, Human (B lymphoid kinase, B lymphoid tyrosine kinase)

Cat no: B1900-01A

Supplier: United States Biological
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Blk, also known as B lymphoid kinase, is a 55 kd tyrosine kinases with a SH3, SH2 and catalytic domains that contain consensus sequences of the src protein tyrosine kinase family. Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells (1). Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk (2). Upon activation, the SH2 domain of Blk binds more than 10 distinct phosphoprotein including those of 72, 76, 115, and 130 kd (3). Blk prefers peptide substrates of the structure I/L-Y-D/E-X-L which resemble critical features of the ITAM motifs found in, e.g. the intracellular components Ig-alpha and Ig-beta of the beta cell receptor. Blk has a strong preference for a negatively charged amino acid in position +1 (4). Source: Recombinant full-length human BLK containing N-terminal GST tag expressed by baculovirus in Sf 9 insect cells. Specific Activity: 579nmol phosphate incorporated into Poly(Glu-Tyr) per minute/mg protein at 30C using a final concentration of 50uM ATP (0.83uCi/assay). Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B1900-01A
Size: 5ug
Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Purity: (same/more than)90% (SDS-PAGE and Coomassie blue staining)
References: 1. Dymecki SM, Niederhuber JE, Desiderio SV. Specific expression of a tyrosine kinase gene, blk, in B lymphoid cells. Science. 1990 Jan 19;247(4940):332-6. 2. Burkhardt AL, Brunswick M, Bolen JB, Mond JJ. Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7410-4. 3. Malek SN, Desiderio S. SH2 domains of the protein-tyrosine kinases Blk, Lyn, and Fyn(T) bind distinct sets of phosphoproteins from B lymphocytes. J Biol Chem. 1993 Oct 25;268(30):22557-65. 4. Schmitz R, Baumann G, Gram H. Catalytic specificity of phosphotyrosine kinases Blk, Lyn, c-Src and Syk as assessed by phage display. J Mol Biol. 1996 Aug 2;260(5):664-77.

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