Bone morphogenic protein 6 (BMP-6) is a member of the TGF-beta superfamily of cytokines regulating homeotic gene expression, embryonic development and neurogenesis. Pre-incubation with amino-terminal peptide 1-29 of BMP-6 inhibits binding of the monoclonal to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP-7, BMP-4, BMP-3 and BMP-2 as well as irrelevant peptides does not inhibit specific tissue staining.
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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