Home  >  Products  >  BOB1 (POU2AF1, POU Domain Class 2-associating Factor 1, B-cell-specific Coactivator OBF-1, BOB-1, OCA-B, OCT-binding Factor 1, OBF1)

BOB1 (POU2AF1, POU Domain Class 2-associating Factor 1, B-cell-specific Coactivator OBF-1, BOB-1, OCA-B, OCT-binding Factor 1, OBF1)

Cat no: B2525-01B


Supplier: United States Biological
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A novel 35kD protein designated Bob1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct-1 and Oct-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2.5-5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Raji cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2525-01B
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human Bob1. Species Crossreactivity: mouse and rat.

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