Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant

Cat no: B2553-06A

Supplier: United States Biological

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Belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. Recombinant human Bone Morphogenetic Protein-2 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids. Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His. Biological Activity: The ED50 as determined by the cytolysis of MC3T3-E1 cells is <50ng/ml corresponding to a specific activity of 2x10e4 IU/mg. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a standard solution of BMP2 as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 100mM acetic acid. Add 0.1% HSA or BSA for long term storage. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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Catalogue number:	B2553-06A
Size:	2ug
Form:	Supplied as a powder lyophilized from 10mM sodium citrate, pH 3.5. Reconstitute with sterile 100mM acetic acid to (same/more than)0.1mg/ml. Add 0.1% HSA or BSA for long term storage. This solution can then be diluted into other aqueous solutions.
Purity:	(same/more than)95% by RP-HPLC and SDS-PAGE Silver Stain. Chromatographically purified.
References:	1. Reppe, S., et al., Biochem. Biophys. Res. Commun. 324(1): 218-223 (2004). 2. Kim, Y.J., et al., J. Biol. Chem. 279(49): 50,773-50,780 (2004). 3. Seeherman, H.J., et al., J. Bone Joint Surg. Am. 86-A(9): 1961-1972 (2004). 4. Kaden, J.J., et al., J. Heart Valve Dis. 13(4): 560-566 (2004). 5. Mummaneni, P.V., et al., J. Neurosurg. Spine 1(1): 19-23 (2004). 6. Wajih, N., et al., J. Biol. Chem. 279(41): 43,052-43,060 (2004).

Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant

Cat no: B2553-06A

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