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Bone Morphogenetic Protein 7 (BMP 7, BMP7, BMP-7, Osteogenic Protein 1, OP 1, OP1, OP-1)

Bone Morphogenetic Protein 7 (BMP 7, BMP7, BMP-7, Osteogenic Protein 1, OP 1, OP1, OP-1)

Cat no: B2553-33Q

Supplier: United States Biological
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Bone morphogenetic protein-7 (BMP-7), also known as osteogenic protein-1, is a member of the TGF- superfamily, and is widely expressed during embryonic development. Like other members of the bone morphogenetic protein family, BMP-7 works synergistically with BMP-2 to play a key role in the differentiation of mesenchymal cells into bone and cartilage tissues by actively recruiting stem cells from the surrounding tissue, thereby initiating the bone formation cascade. In adult cells, BMP-7 is predominately expressed in the brain, kidneys, and bladder. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml detected BMP-7 (Pro-peptide) in 10ug of human kidney tissue lysate. Immunohistochemistry (Paraffin): 1:200 was used to detect BMP-7 (pro-peptide) in human kidney tissue. Requires Citrate pH6.0 retrieval. Optimal dilutions to be determined by the researcher. Positive Control: human kidney tissue or lysate. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2553-33Q
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Mab
Isotype: IgG1,k
Purity: Purified
References: 1. Gregory, K.E., et al. (2005). Journal of Biol Chem. 280(30):27970-27980.
Additional info: Recognizes the pro-peptide form of human BMP-7 at ~31kD.

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