Bordetella lipopolysaccharides (LPS) are characterised by the absence of the O-polysaccharide (O-antigen) but have an extended core region and are more commonly termed as LOS. B. pertussis is a non-motile, gram-negative bacteria that is the common causative agent for whooping cough. B. bronchiseptica is a closely related species that is often involved in respiratory tract infection in smaller mammals.
Applications:
Suitable for use in ELISA, Immunofluorescence, and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:20-1:200
Western Blot: 1:10-1:50
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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