BORIS (Brother of the Regulator of Imprinted Sites)

Cat no: B2560-35

Supplier: United States Biological

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BORIS (Brother of the Regulator of Imprinted Sites) also known as CCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm. Cellular Localization: Nuclear and cytoplasmic Positive Control: Human prostate. Also found in cancerous cell lines. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Detects a band of ~ 75kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	B2560-35
Reactivities:	Human, Canine
Hosts:	Rabbit
Applications:	ELISA, Western Blot
Size:	100ug
Form:	0.02M Potassium phosphate, 0.15 M Sodium chloride pH7.2
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
References:	Klenova EM et al. The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer. Semin Cancer Biol 12:399-414 (2002). <PUBMED:12191639> Loukinov DI et al. BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma. Proc Natl Acad Sci U S A 99:6806-11 (2002). <PUBMED:12011441>
Additional info:	Species Crossreactivity: Crossreacts with Human. Expected to cross react with chimpanzee. May react with mouse, canine and rat sources based on sequence homology.

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BORIS (Brother of the Regulator of Imprinted Sites)

Cat no: B2560-35

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