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BR Serine/Threonine Protein Kinase 1 (BRSK1, SAD1 Kinase, Serine/Threonine Kinase SAD-B)

BR Serine/Threonine Protein Kinase 1 (BRSK1, SAD1 Kinase, Serine/Threonine Kinase SAD-B)

Cat no: B2673-75C

Supplier: United States Biological
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BRSK1 is a widely expressed protein, with highest levels being detected in the brain and testis. It is essential for the polarization forebrain neurones which results in the distinct properties of axons and dendrites. Research suggests that BRSK1 is involved in the regulation of G2/M arrest and functions as a checkpoint kinase following UV- or methyl methane sulfonate (but not IR-) induced DNA damage. BRSK1 phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. Multiple isoforms of BRSK1 have been identified. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (Paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies Sheep Anti Rabbit IgG HRP Immunohistochemistry Positive Control Tissue: Human brain Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B2673-75C
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Kishi M, et al. (2005) Mammalian SAD kinases are required for neuronal polarization. Science 307:929-32. 2. Lu R, et al. (2004) Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. J. Biol. Chem. 2004; 279:31164-70.
Additional info: Recognizes human BR serine-threonine kinase 1 in human brain tissue lysates at ~87kD. Species crossreactivity: Mouse, Rat.

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