Home  >  Products  >  Brachyury (Brachyury Homolog, Brachyury Protein, Bry, MGC104817, T, T Protein, TFT)
Brachyury (Brachyury Homolog, Brachyury Protein, Bry, MGC104817, T, T Protein, TFT)

Brachyury (Brachyury Homolog, Brachyury Protein, Bry, MGC104817, T, T Protein, TFT)

Cat no: B2674-75F

Supplier: United States Biological
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Brachyury is a 435aa containing embryonic nuclear transcription factor binding to the palindromic T-site. It binds to the T-box in the N-terminus to regulate the genes required for mesodermal differentiation. It is the earliest indicator of mesoderm formation and differentiation. Brachyury is expressed during gastrula and neurula stages in involuting mesoderm and in the notochord. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2674-75F
Reactivities: Human, Non-Human Primate
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Technau, U. Bioessays. 23:788-794 (2001). 2. Cho, J.Y. et al. Int. Immunol. 15:1149-1160 (2003). 3. Palena, C. et al. Clin. Cancer Res. 13:2471-2478 (2007).
Additional info: Recognizes human Brachyury. Species Crossreactivity: chimpanzee, rhesus monkey. Species sequence homology: mouse, rat

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