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Brain Derived Neurotrophic Factor (BDNF) BioAssay(TM) ELISA Kit

Brain Derived Neurotrophic Factor (BDNF) BioAssay(TM) ELISA Kit

Cat no: B2700-08

Supplier: United States Biological
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Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. Kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. With the BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin, enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration, i.e., the higher the OD the higher the BDNF concentration in the sample. Sensitivity: 7.8pg/ml Range of Detection: 7.8-500pg/ml Crossreactivity: No significant crossreactivity with NGF, NT4/5 or NT3 Intra-assay Variation:
Catalogue number: B2700-08
Applications: ELISA
Size: 2Plates
References: 1. Laske, C., et al. (2007) Increased BDNF serum concentration in fibromyalgia with or without depression or antidepressants. Journal of Psychiatric Research. 41:600-605. 2. Tramontina, J., et al. (2007) Val66met polymorphism and serum brain-derived neurotrophic factor levels in bipolar disorder. Molecular Psychiatry. 12:230- 231. 3. Neumeister, A., et al., (2005) Effects of Tryptophan Depletion on Serum Levels of Brain-Derived Neurotrophic Factor in Unmedicated Patients with Remitted Depression and Healthy Subjects. Am. J. Psychiatry. 162:805-807.

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