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Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)

Cat no: B2700


Supplier: United States Biological
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BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kD homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and mouse BDNF sequences are identical. Biological Activity: Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1ug/ml. AA Sequence: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
Catalogue number: B2700
Size: 2ug
Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
Purity: (same/more than) 98% (SDS-PAGE, HPLC) Endotoxin: (same/less than) 0.1ng/ug (1EU/ug)
References: US Biological application reference: Connolly, A.M. et al., (2006) Biological Psychiatry 59:354-363 (E).

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